Journal: Advanced Science

Article Title: CD38‐Specific Gallium‐68 Labeled Peptide Radiotracer Enables Pharmacodynamic Monitoring in Multiple Myeloma with PET

doi: 10.1002/advs.202308617

Figure Lengend Snippet: Pharmacokinetics of [ 68 Ga]Ga‐AJ206 in NSG mice bearing MOLP8 tumor xenografts. A) Coronal sections of the fused dynamic PET/MR images showing [ 68 Ga]Ga‐AJ206 distribution. Primary tumor is indicated by the dashed white circle. Mice were intravenously injected with ≈ 9.25 MBq (≈250 µCi) [ 68 Ga]Ga‐AJ206; L, Liver; B, Bladder. B) Time–activity curves of [ 68 Ga]Ga‐AJ206 in the kidney, heart, liver, tumor and muscle derived from images A. Data in panel B is represented as mean ± SEM ( n = 3). C) Uptake of [ 68 Ga]Ga‐AJ206 in tumor, blood, liver and muscle derived from ex vivo biodistribution study. D) Tumor‐to‐muscle and tumor‐to‐blood ratios derived from biodistribution data. Data in panels C and D are from mice intravenously injected with ≈ 2.96 MBq (≈80 µCi) [ 68 Ga]Ga‐AJ206 and sacrificed at different time‐points after injection, represented as mean ± SEM ( n = 4 or 5).

Article Snippet: MOLP8 cells were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Germany, under the material transfer agreement guidelines with Johns Hopkins University.

Techniques: Drug discovery, Positron Emission Tomography-Magnetic Resonance Imaging, Injection, Activity Assay, Derivative Assay, Ex Vivo

Journal: Advanced Science

Article Title: CD38‐Specific Gallium‐68 Labeled Peptide Radiotracer Enables Pharmacodynamic Monitoring in Multiple Myeloma with PET

doi: 10.1002/advs.202308617

Figure Lengend Snippet: Kinetics and distribution of [ 68 Ga]Ga‐AJ206 in mice with MOLP8 tumor xenografts; data is presented as mean ± SEM ( n = 4 or 5) of %ID g −1 .

Article Snippet: MOLP8 cells were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Germany, under the material transfer agreement guidelines with Johns Hopkins University.

Techniques:

Journal: Advanced Science

Article Title: CD38‐Specific Gallium‐68 Labeled Peptide Radiotracer Enables Pharmacodynamic Monitoring in Multiple Myeloma with PET

doi: 10.1002/advs.202308617

Figure Lengend Snippet: Validation of [ 68 Ga]Ga‐AJ206 specificity for CD38 in disseminated MM disease models and primary plasma cell leukemia xenografts. A) IVIS‐bioluminescence image of luciferase expressing MM1S disseminated tumor model. B) In vivo uptake of [ 68 Ga]Ga‐AJ206 in lungs and bones in MM1S‐Luc bearing mice. C) Flow cytometry analysis of CD38 expression in lungs harvested from MM1s‐Luc cells injected mice. Lungs from PBS treated mice were used as controls (top panel). IHC staining of bones shows CD38 expression (bottom panel). D) Rendered in vivo and ex vivo [ 68 Ga]Ga‐AJ206‐PET images of lower limbs of MOLP8 injected animals. E) IHC images of MOLP8 bone marrow tumor and PBS‐treated animals confirmed CD38 expression. F) Quantification of PET signal (%ID/cc) in the bone marrow of PBS, MM1S‐Luc and MOLP8 injected animals. G) Tumor/muscle (T/M) ratios of PET measures in the bone marrow of PBS, MM1S and MOLP8 injected animals. H) Flow cytometry analysis of CD38 expression in cells extracted from the bone marrow of PBS, MM1S‐Luc and MOLP8 cell injected mice. Data in figure F and G are shown as box and whisker plots showing all data points ( n = 7 in PBS, n = 5 in MM1S and n = 8 in MOLP8). Ordinary one‐way ANOVA using multiple comparison test. **, P ≤ 0.01; *** P ≤ 0.001; ****, P ≤ 0.0001.

Article Snippet: MOLP8 cells were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Germany, under the material transfer agreement guidelines with Johns Hopkins University.

Techniques: Biomarker Discovery, Clinical Proteomics, Luciferase, Expressing, In Vivo, Flow Cytometry, Injection, Immunohistochemistry, Ex Vivo, Whisker Assay, Comparison